Skip to main content
. 2021 Jun 8;30(1):448–467. doi: 10.1016/j.ymthe.2021.06.006

Figure 1.

Figure 1

Upregulated VN1R5 expression was related to cisplatin resistance in HNSCC cells

(A) Microarray analysis of cisplatin-sensitive and -resistant HNSCC cells. VN1R5 was upregulated in cisplatin-resistant HNSCC cells. (B) VN1R5 expression was measured by qPCR in cisplatin-sensitive and -resistant HN4 and HN30 cells. (C) VN1R5 expression was measured by western blotting in cisplatin-sensitive and -resistant HN4 and HN30 cells. (D) VN1R5 expression was measured by qPCR in TPF-sensitive and -resistant HNSCC tissues. (E) 5-year overall survival rates of HNSCC patients with different VN1R5 expression levels. (F) Compared with NC-transfected cells (black line), HN30/DDP cells with VN1R5 downregulation were sensitized to cisplatin (blue line). The IC50 values are shown on the right. (G) Compared with NC-transfected cells (black line), HN30 cells with VN1R5 upregulation exhibited cisplatin resistance (red line). The IC50 values are shown on the right. (H) Under intraperitoneal injection with 5 mg/kg cisplatin every 3 days 5 times, the volumes and weights of tumors in nude mice subcutaneously inoculated with KO-VN1R5 HN30/DDP cells at the end of the experiment are shown (n = 6/group). (I) The tumor volumes of the KO-VN1R5/KO-NC groups were calculated every 5 days. The mice were intraperitoneally injected with 5 mg/kg cisplatin at days 6, 9, 12, 15, and 18 after tumor inoculation. (J) Under intraperitoneal injection with 5 mg/kg cisplatin every 3 days 5 times, the volumes and weights of tumors in nude mice subcutaneously inoculated with HN30 cells stably transfected with LV-VN1R5 at the end of the experiment are shown (n = 6/group). (K) The tumor volumes of the LV-VN1R5/LV-NC groups were calculated every 5 days. The mice were intraperitoneally injected with 5 mg/kg cisplatin at days 6, 9, 12, 15, and 18 after tumor inoculation. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Error bars, means ± SDs. (TPF, docetaxel+cisplatin+5-fluororacil; NC, negative control; LV, lentiviral vector; KO, knockout; i.p., intraperitoneal.)