Figure 6.

lnc-POP1-1 bound to MCM5 to inhibit the ubiquitination of MCM5

(A) The grayscale values of MCM5 protein expression affected by MG132 and the relative intensity are shown. (B) Western blot of the effect of lnc-POP1-1 on MCM5 protein degradation in the presence of 200 μM CHX. (C) The protein levels of MCM5 in nuclear and cytoplasmic fractions were analyzed by western blotting of HN4/DDP and HN30/DDP cells transfected with SS-lnc-POP1-1. (D) The protein levels of MCM5 in nuclear and cytoplasmic fractions were analyzed by western blotting of HN4 and HN30 cells transfected with LV-lnc-POP1-1. (E) Western blot analysis of the ubiquitination of MCM5 in HN4/DDP and HN30/DDP cells transfected with SS-lnc-POP1-1 and treated with 10 μM MG132 for 8 h. (F) Western blot analysis of the ubiquitination of MCM5 in HN4 and HN30 cells overexpressing lnc-POP1-1 and treated with 10 μM MG132 for 8 h. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. Error bars, means ± SDs. (Ub, ubiquitin; IP, immunoprecipitation; IB, immunoblotting.)