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. 2021 Oct 31;20(2):399–408. doi: 10.1111/pbi.13723

Figure 4.

Figure 4

AcrIIA4 prevents dCas9‐based activation of NbDFR and NbAN2 endogenous genes in Nicotiana benthamiana. N. benthamiana leaves expressing dCas9EV2.1 and the corresponding specific or unspecific gRNAs for the activation of NbDFR or NbAN2, with or without NLS‐AcrIIA4. L1, L2 and L3 indicate independent agroinfiltrated leaves. Error bars indicate SD (n = 3, technical replicates). Statistical analyses were performed using one‐way ANOVA (Tukey's multiple comparisons test, P ≤ 0.05).