Figure 6.

The effect of NYC3, a regulator of chlorophyll degradation, on rice ShB resistance and yield‐related traits. (a) NYC3 is more significantly induced by Rhizoctonia solani (R. solani) in variety 9522 than in YSBR1. Data are shown as means ± SD. The experiment was repeated three times with similar trends among each other. (b,c) NYC3‐RI and NYC3‐OX alter rice resistance to R. solani in detached leaf (b) and whole plant (c) inoculations. All transgenic lines are in the 9522 variety. Images were taken and data collected at 6 and 8 DPI, respectively. Scale bar, 2 cm in (b); 5 cm in (c). (d) DAB and TB staining for H₂O₂ accumulation and cell death of NYC3‐RI, NYC3‐OX and wild type 9522 at 3 DPI. Scale bar, 1 cm in left, 100 μm in right. (e–h) Effects of NYC3 silencing on the yield‐related agronomic traits. Pictures displayed the morphology of whole plant (e), grain length and width (f). (g) shown the 1000 grain weight and yield per plant, (h) shown the yield test. Scale bar, 10 cm in (e); 1 cm in (f). All data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ns, not significant using Student’s t‐tests.