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Quantification of protein induction by nitrogen in maize leaves. Protein extracts from the youngest, fully expanded leaves of 11‐day‐old plants grown in different forms and amounts of nitrogen were measured with ELISA. Concentrations of ZmLOX6, ZmPEPC and ZmPPDK in the total extractable leaf protein were 0.85%, 4.8% and 5.1%, respectively, in the plants supplemented with 10 mM KNO3. For fold expression, base level for each of the three proteins is set to 1.
