Figure 5.

Quantification of leaf ZmLOX6 protein in transgenic and control maize plants by ELISA. ZmLOX6 gene was transformed under the control of different promoters with or without a vacuolar‐targeting signal (VTS) from maize aleurain. Protein extracts from the youngest, most fully expanded leaf of 11‐day‐old plants grown in 5 mM potassium nitrate were subjected to ELISA with the anti‐ZmLOX6 antibody. A subset of representative events corresponding to the numbers on the abscissa with varying ELISA signal were probed with the anti‐ZmLOX6 antibody on a Western blot (inset). The constructs with each of the three promoters with or without the VTS are colour coded. Protein level displayed on the ordinate is expressed as percent of the total extractable leaf protein.