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. 2021 Dec 8;20(2):374–389. doi: 10.1111/pbi.13720

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© 2021 Pioneer Hi-Bred International, Inc (Corteva Agriscience). Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Remobilization of ZmLOX6 and ZmPEPC proteins after pollination from the plants overexpressing ZmLox6 under the control of the pZmPEPC promoter. Ear leaves of field‐grown 16 independent transgenic events overexpressing ZmLOX6 under the control of the pZmPEPC promoter and the parental nontransgenic line were repeatedly sampled at different days after pollination (DAP). The protein extract was subjected to ELISA for the ZmLOX6 and ZmPEPC proteins. The suffix TG after ZmLOX6 and ZmPEPC refers to ZmLOX6‐overexpressing transgenic plants (green font) and the suffix C refers to nontransgenic control plants (black font). The ELISA‐quantified proteins are expressed as a percentage of the total extractable leaf proteins at each stage.