Figure 10.

Relationship of ZmLOX6 protein in pZmPEPC‐driven ZmLOX6 transgenic events with grain yield. ZmLOX6 protein is presented as a percentage of the total leaf protein in the ear leaf 2 weeks before flowering in Woodland, CA (2008) (a), and its relationship to grain yield under drought stress at flowering (b). The ear leaf was at the 8^th position of the aboveground nodes. Leaf discs were collected as described in Experimental Procedures and frozen immediately in dry ice. The leaf extracts were subjected to ELISA using the anti‐LOX6 antibody. One of the ten transgenic events, #34, failed in 2008 so was omitted from the graph. Another event, #1, was apparently completely silenced as it had the same level of ZmLOX6 as the construct null. Six of the transgenic events had similar concentration of the ZmLOX6 protein but two had significantly lower concentration, although they still accumulated three times as much protein as the construct null. Grain yield under drought stress was positively correlated with the ZmLOX6 protein concentration measured before the application of stress. ZmPEPC protein, which was measured on the same extracts as for the ZmLOX6 protein, is shown for reference. Its levels were unaffected by those of ZmLOX6.