Figure 3.
Systemic delivery of human KitStop ESO inhibits tumor growth in a humanized xenograft MC neoplasia model
(A) Schematic representation of the protocol design for the humanized xenograft model used. (B) Examples of tumors in NSG female mice that were injected with 1 × 106 HMC-1.2 cells into a primary site at the right flank subcutaneously. The red arrows indicate the tumor and the green arrows show a secondary growth caused by drag of the needle during inoculation. (C) Measurements of tumor volume over time during treatment (n = 5 mice per group). Arrows indicate days that KitStop or vehicle control was administered. (D) Photograph of tumors after mice were euthanized at day 14. Measurements are in centimeters. (E) Weight of excised tumors after mice were euthanized on day 14. (F) Spleen weight at the conclusion of the experiment. (G) Liver weight at the conclusion of the experiment. (H) Weight of mice was monitored during the course of the experiment. No significant differences were observed during the course of the experiment. Data are the mean ± SEM from five mice per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, using ANOVA with Dunnett’s post-test (B) or an unpaired t test (D and E).