Figure 5.

miR-142a-3p alleviates DSS-induced colitis by promoting the growth of L. reuteri

(A) L. reuteri was grown in culture media with 0.5 μM miR-142a-3p mimics and scramble. Growth was monitored as absorbance at 600 nm every 2 h (n = 3). (B) Schematic diagram of the putative binding sites of the seed sequence of miR-142a-3p in the two genes (LREU_RS06530 and LREU_RS03575) of L. reuteri. (C) Relative expression of the two genes of L. reuteri incubated with miR-142a-3p mimics (n = 3). (D–G) The effects of miR-142a-3p on microbiota composition in DSS-treated mice (n = 3–4). (D) Principal coordinates analysis (PCoA) based on weighted UniFrac distance. (E) Relative abundance of bacteria classified at a species-level taxonomy. (F) Relative abundance of L. reuteri determined by qRT-PCR. (G) Quantification of reuterin in feces by HPLC. (H and I) The effects of oral administration of L. reuteri (1 × 10¹¹ colony-forming units [CFU]/mL) on DSS-induced colitis (n = 6). (H) Body weight change. (I) Disease activity index. Data were analyzed with one-way ANOVA. Values are mean ± SEM. ∗p < 0.05.