Figure 1.

Exercise-induced ADAR2 promotes cardiomyocyte proliferation and survival in vitro

(A) Quantitative real-time PCR and representative western blot showing expression change of ADAR2 in swimming-trained mice hearts versus control mice hearts (n = 6, ∗∗p < 0.01). (B) Representative western blot testing expression change of ADAR2 after overexpression (n = 6, ∗∗p < 0.01). (C) Representative western blot testing expression change of ADAR2 after siRNA strand-3 knockdown (n = 6, ∗∗p < 0.01). (D) Analysis of proliferation markers, including EdU (red) and Ki67 (red), after knockdown or overexpression of ADAR2. α-actinin (green) and DAPI (blue) are co-stained (n = 6, ∗∗p < 0.01); scale bar, 50 μm. (E and F) Analysis of apoptosis by TUNEL staining (E) and western blot of Bax, Bcl2, caspase 3, and cleaved-caspase 3 (F) after ADAR2 overexpression or ADAR2 knockdown in doxorubicin (DOX)induced apoptosis model (n = 6, ∗p < 0.05, ∗∗p < 0.01).