Figure 8.

C/EBPβ acts as an upstream regulator of ADAR2

(A) Schematic illustration of the JASPAR-predicted binding sites of C/EBPβ in the promoter region of Adar2. (B) ChIP-PCR assay showed that C/EBPβ could bind to the ADAR2 promoter region (n = 4, ∗∗p < 0.01). (C) Luciferase assay revealed the negative regulation of C/EBPβ on ADAR2 (n = 6, ∗∗p < 0.01). (D) Quantitative real-time PCR analysis of ADAR2 expression with or without C/EBPβ knockdown (n = 6, ∗∗p < 0.01). (E) Representative images of immunofluorescence staining and quantification of the EdU⁺ cardiomyocytes displayed the rescue effect of ADAR2 overexpression on C/EBPβ overexpression (n = 6, ∗∗p < 0.01); scale bar, 50 μm. (F) Representative images of immunofluorescence staining and quantification of the TUNEL⁺ cardiomyocytes displayed the rescue effect of ADAR2 overexpression on C/EBPβ overexpression in DOX-induced NRCMs apoptosis (n = 6, ∗p < 0.05, ∗∗p < 0.01); scale bar, 50 μm.