FIG. 1.

Isolation of the APV M gene and production of recombinant protein. The M gene was isolated from pCR-XL-TOPO by PCR and subcloned into the pQE-30 expression plasmid. A two-step process was used to purify M proteins, a nickel-nitriolotriacetic acid column that binds the His₆, tag located at the NH₂ terminus of the protein and gel purification. (A) PCR product of APV/CO (lane 1) and APV/MN2A (lane 2) in an agarose gel. (B) Recombinant M protein from APV/CO (lane 1) and APV/MN2A (lane 2) in an SDS-polyacrylamide gel stained with Coomassie blue.