FIG. 2.

Western blot analysis of APV-positive and -negative turkey sera with APV proteins and recombinant M proteins. The partially purified APV proteins and the recombinant M protein were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane. Each lane of the membrane was incubated with a 1:40 dilution of turkey serum followed by horseradish peroxidase-conjugated anti-turkey IgG (1:20,000 dilution) and detected by chemiluminescence. (A) Reactivity of the sera with APV proteins; (B) reactivity with recombinant M protein. Lanes 1 to 8 show the reactivity with positive turkey sera, and lanes 9 and 10 show the reactivity with negative sera.