FIG. 5.

Comparison of the sensitivity of M protein ELISA with routine APV ELISA for detection of APV-specific IgGs from experimentally infected turkeys. Turkeys (n = 34) were experimentally inoculated with live APV (APV/MN1A), and serum was collected 4 weeks later. A 1:40 dilution of each sample was applied to plates coated with recombinant M protein or APV-infected cell lysate and detected using horseradish peroxidase-conjugated goat anti-turkey IgGs. The A₄₉₀ values from M protein-coated plates were consistently higher than those from cell lysate-coated plates (P < 0.001).