Skip to main content
. 2021 Aug 25;30(1):431–447. doi: 10.1016/j.ymthe.2021.08.027

Figure 5.

Figure 5

The stability of PTEN is regulated by circMRPS35 and STX3 in HCC cells

v(A) Quantitative real-time PCR assays of STX3 in STX3 silenced or control Huh-7 and HCC-LM3 cells. (B) Western blot analysis of STX3 and PTEN in STX3 silenced or control Huh-7 and HCC-LM3 cells. (C) IP by anti-IgG, anti-PTEN, or anti-STX3 antibodies and western blot analysis of PTEN and STX3 in Huh-7 and HCC-LM3 cells. (D) 293T cells were transfected and treated with/without MG132, and then cells were immunoprecipitated with anti-FLAG antibodies and western blot by indicated antibodies. (E) STX3 silenced or control Huh-7 and HCC-LM3 cells were treated with MG132; cells were immunoprecipitated with anti-PTEN antibodies and western blot by indicated antibodies. (F) circMRPS35 KD or control Huh-7 and HCC-LM3 cells were treated with MG132; cells were immunoprecipitated with anti-PTEN antibodies and western blot by indicated antibodies. (G and H) circMRPS35 KD or control Huh-7 and HCC-LM3 cells were treated with CHX for indicated times; western blot and quantification of relative PTEN protein levels are shown. Error bars represent the means ± SEM of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.