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. 2022 Jan 11;10(1):e003735. doi: 10.1136/jitc-2021-003735

Figure 4.

Figure 4

Human anti-PD-1 IgG4-FALA enhances endogenous CD8 T-cell responses to OVA in hFcγR-expressing mice. Human FcγR-expressing mice received (i.p) 5 mg OVA alone or in combination with 200 µg anti-CD40 (mIgG1), 200 µg anti-CD40 plus 250 µg irrelevant mAbs (AT171-2 hIgG4 or AT171-2 hIgG4-FALA; in-house) or 200 µg anti-CD40 plus 250 µg anti-PD-1 mAbs (EW1-9 hIgG4 or hIgG4-FALA). (A, B) Percentage of (A) and PD-1 expression in (B) SIINFEKL-specific CD8 T cells at day 7. (C, D) Expression of PD-1 (C) and frequency of CD44+ cells in tetramer negative CD8 T cells at day 7. Representative of two independent experiments, N=4 mice per group. Bars represent mean±SD, *p<0.05, **p<0.01, (One-way ANOVA). ANOVA, analysis of variance; FALA, F234A-L235A mutation; FcγR, Fcγ receptors; PD-1, programmed cell-death.