Figure 5.

GDNPs combined with PD-1 mAb therapy enhance long-term antigen-specific anti-tumor memory

(A) Survival curves for Vehicle, PD-1 mAb, GDNPs, and Combo groups in the CT26 murine colon tumor model (n = 10 for each group, p = 0.0132). (B) Paradigm of tumor rechallenge assay and time schedule. (C) Quantification of tumor volumes for tumor rechallenge assay. Representative pictures for tumor-bearing mice (n = 6 for each group, ∗∗∗∗p < 0.0001). (D) Quantification of CD44⁺ CD62L⁺ and CD44⁺ CD62L⁻ for CD4⁺ or CD8⁺ T cells in the spleen of wild-type (WT) or Combo pre-treated mice (n = 5 for each group, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001). Data are presented as mean ± SEM and analyzed using two-way ANOVA, Student’s t test, or log rank (Mantel-Cox) test.