Figure 1.

RITDM gene editing strategy

(A) A RITDM system consists of a programmable DLR molecule containing a sequence-specific recognition domain (D), such as a zinc-finger array, fused to a linker domain (L), and a non-sequence-specific DNA binding domain (R). (B) Schematic of RITDM editing. A locus-specific DLR molecule binds DNA at the target site to block replication fork progression temporarily, and a DNA correction template anneals and incorporates at the DNA replication fork. The resulting intermediary DNA heteroduplex can be repaired to enable a permanent genetic modification.