Skip to main content
. 2021 Dec;10(12):3237–3247. doi: 10.21037/tp-21-527

Figure 5.

Figure 5

miR-143-3p targets 3’UTR of HK2 mRNA in neuroblastoma cells. (A) Prediction of the miR-143-3p-HK2 3'-UTR interaction from starBase 2.0. (B) Expressions of miR-143-3p in neuroblastoma and adjacent noncancerous tissues (n=40) was examined by qRT-PCR. (C) Expressions of miR-143-3p in human primary neuron cell and neuroblastoma cells detected by qRT-PCR. (D) The correlation between miR-143-3p and HK2 mRNAs from neuroblastoma tissues was analyzed by Pearson correlation test. (E) IMR-32 and SK-N-SH cells were transfected with control miRNA or miR-143-3p for 48 h, protein expressions of HK2 were examined by western blot. (F) Luciferase assay was performed in IMR-32 and SK-N-SH cells which were co-transfected with luciferase vector containing WT- or Mut- HK2 3'-UTR plus control miRNA or miR-143-3p. **, P<0.01; ***, P<0.001. qRT-PCR, quantitative reverse transcription polymerase chain reaction; mRNA, messenger RNA; miRNA, microRNA; WT, wild type.