Fig. 2.

Rg3-RGE induces mitophagy in lung cancer cells. (A) A549 and H460 cells were treated with the indicated concentrations of Rg3-RGE for 24 h, and levels of LC3-II and p62 were detected by western blotting. (B) A549 and H460 cells were incubated with the indicated concentrations of Rg3-RGE for 24 h. Cell morphology was observed under a light microscope. (C) After the indicated concentration of RG3-RGE for 24 h, A549 and H460 cells were immunostained with anti-LC3II antibody. (D) A549/H640 cells were co-treated with chloroquine (50 μM) for 24 h, and levels of p62 and LC3II were measured by western blotting. (E) A549 and H460 cells were treated with the indicated concentration of Rg3-RGE for 24h, and mitochondrial protein levels (MFN2, OPA1) were detected by western blotting. (F) A549 and H460 cells were treated with Rg3-RGE (200 μg/ml) for 24 h. Mitochondrial colocalization with LC3II was detected by a confocal microscope.