Figure 6.

Tracking the ciCPCs implanted in infarcted hearts

(A) Schematic representation of using Nkx2.5^Cre/Rosa^{RFP(tdTomato)} TTF-derived ciCPCs to determine cell fusion in GFP reporter mice or identify cell differentiation in wild-type mice. IRES, internal ribosome entry site. Scale bars, 200 μm. (B and C): Representative imaging of RFP⁺ cell retention in GFP myocardium after injection. Scale bars, 100 μm. (D–F) Representative imaging of cTnT⁺ CMs, αSMA⁺ SMCs, or CD31⁺ ECs derived from RFP⁺ ciCPCs at 4 weeks post MI. White arrows indicate the integration of ciCPCs with the host's microvessels. Scale bars, 100 μm. (G) Overall differentiation efficiency of ciCPCs in infarcted hearts. See also Figure S7.