Figure 5.

The bifunctional LVs AS3m.C and AS3m.13bpdel reduce RBC sickling

In vitro sickling assay measuring the proportion of sickled RBCs under hypoxic conditions (0% O₂). We reported the percentage of sickling RBCs (left panel) and representative photomicrographs of RBCs derived from control (Ctr), LVs AS3m.C and AS3m.13bpdel samples that were either mock transfected or transfected with Cas9-GFP (right panel; 2 SCD donors). Arrows indicate sickling cells. Data are expressed as mean ± SEM. Scale bar, 20 μm (upper left).