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. 2022 Jan 11;23:5. doi: 10.1186/s12931-022-01926-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Primary culture and multiple differentiation of ADSCs. These spindle-shaped cells isolated from human adipose tissue adhered to the Petri dishes and formed a monolayer (A). ADSCs differentiated into adipocytes as lipids were accumulated in ADSCs culture. Black arrows indicate the presence of lipids droplets (B). ADSCs differentiated into osteoblasts as extracellular calcium phosphate deposits were observed in ADSCs culture. White arrows indicate the presence of osteogenic mineral deposition (C). ADSCs differentiated into chondroblasts as nodules were formed in ADSCs culture. Red arrows indicate the presence of nodules and inter chondrogenic nodule connections (D). ADSCs were positive for CD44 (E). ADSCs were positive for CD90 (F)