Fig. 4.

The regulation of Notch4 on the protein levels associated with proliferation, apoptosis and migration through ERK, JNK, or P38 MAPK pathway. A Protein levels of survivin, PCNA, Bax, Bcl-2, ERK, p-ERK, MMP2, and Notch4 were determined in HPASMCs transfected with negative control plasmid or Notch4 plasmid together with U0126 for 24 h. Bars show mean fold change of vector or as indicated. (n = 4) B Protein levels of survivin, PCNA, Bax, Bcl-2, JNK, p-JNK, MMP2, MMP9, and Notch4 were determined in HPASMCs transfected with negative control plasmid or Notch4 plasmid together with SP600125 for 24 h. Bars show mean fold change of vector or as indicated. (n = 4) C Protein levels of survivin, PCNA, Bax, Bcl-2, JNK, p-JNK, MMP2, and Notch4 were determined in HPASMCs transfected with negative control plasmid or Notch4 plasmid together with SB203580 for 24 h. Bars show mean fold change of vector or as indicated. (n = 3) Data were presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, comparison with HPASMCs transfected with negative control plasmid; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001, comparison with HPASMCs transfected with Notch4 plasmid. One-way ANOVA followed by Tukey’s correction for post-hoc comparisons were performed for A–C. ERK extracellular signal-regulated kinase, JNK c-Jun N-terminal kinase, MAPK mitogen-activated protein kinase, vector negative control plasmid, Notch4 Notch4 plasmid