Fig. 7.

Notch4 gene silence alleviates pulmonary artery remodeling. A Hematoxylin–eosin staining and immunohistochemistry staining for α-smooth muscle actin, in lung sections of rats treated with AAV1.Notch4 or AAV1.NC under normoxic or hypoxic condition (n = 5–7). Magnification, × 200; Bar, 50 μm. B Notch4 (red) and α-SMA (green) immunofluorescence staining in small pulmonary arteries in rat lung tissues. Nuclei are counterstained with DAPI (blue). Magnification, × 400; Bar, 20 μm. C Protein expression of Notch4, p-ERK, ERK, p-JNK, JNK, p-P38, P38 in pulmonary artery in rats treated with AAV1.Notch4 or AAV1.NC under normoxic or hypoxic condition (n = 4). Data were presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One-way ANOVA followed by Tukey’s correction for post-hoc comparisons were performed for A and C. H&E hematoxylin–eosin staining, α-SMA α-smooth muscle actin, AAV adeno-associated virus, ERK extracellular signal-regulated kinase, JNK c-Jun N-terminal kinase