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. 2022 Jan 12;10(1):e00822-21. doi: 10.1128/spectrum.00822-21

TABLE 2.

Specific amino acid mutations on HA of the representative H9N2 isolated strains

Subgroup Potential antigenic sites
 RBSa
H3 numbering 57 81 96 133 135 137 145 156 158 159 160 183 188 189 190 192 193 199 205 208 216 273 274 275 276 285 306 325 226 227 228
H9 numberingb 66c 90c 107d 145c 147 149c 153c 164c 166 167c 168c 191 196 197 198c 200c 201 207 213d 216d 224d 282d 283d 285d 287d 294d 315d 334d 234 235 236
HA1 residuese 48 72 89 127 129 131 135 146 148 149 150 173 178 179 180 182 183 189 195 198 206 264 265 267 269 276 297 316 216 217 218
Cluster 1 NJ/99-vac H G M N T K D Q S N A N D T T T N D T D M N S N V R P A Q Q G
SD/96-vac H G M S T K D Q D N A N D T A T N D T D V N S N V R P A Q Q G
GD/94-vac H G M S T K D Q S N A N D T A T N D T D V N S N V R P A Q Q G
F/98-vac H G M S T K D Q N N A N D T A T N D T D M N S N V R P A Q Q G
Cluster 2 A2093 H G L S T K D Q N N A N D T V T N D A E L K R S T K S S L Q G
441 H G L S T K D Q N N A N D T V T N D A E L K R N T K S S L Q G
2106 H G L S T K D Q N N A N E T A T N D A E L K R S T K S S L Q G
B469 R E V S T K D Q N N N N D T A T N D A D L K S N T R P A L Q G
B445 H G L S T K D Q N N A N D T A T N D A E L K R S T K S S L Q G
F1650 H G L S T K D Q N N A N E T V T N D A E L K R S T K S S L M G
G755 N G L S T K D Q N N A N E T V T N D A E L K R S T K S S L M G
H97 N G L S T K D Q N N A N E T A T N D A E L K R S T K S S L M G
H1258 H G L S T K D Q N N A N D T V T N D A E L K R S T K S S L M G
H1301 H G L S T K D Q N N A N D T A T N D A E L K R S T K S S L M G
D1021 H G L S T K D Q D N A N D T V T N D A E L K R S T K S S L M G
Cluster 3 D2641 Q E L N T T G R N G D N D T T R D D A E L K R S T K S S L M G
D345 Q E L D T T G R N G D N D T T R N D A E L K R S T K S S L M G
E2290 Q E L D T T G R N G N N D T T R D D A E L K R S T K S S L M G
E552 Q E L D T T G R N G D N D D T R N D A E L K R S T K S S L M G
F2170 Q E L D T T G R N G D N D T T R N D A E L K R S T K S S L M G
G378 Q E L D T T G R N G E N D D T R G D A E L K R S T K S S L M G
G705 Q E L D T T G R N G E N D T T R D D A E L K R S T K S S L M G
G1451 Q E L D T T G R N G N N D D T R N D A E L K R S T K S S L M G
G1586 Q E L D T T G R N G E N D V T R G D A E L K R S T K S S L M G
G1728 Q E L D T T G R D G N N D D T R N D A E L K R S T K S S L M G
G1921 Q E L D T T G R D G N N D T T R G D A E L K R S T K S S L M G
H224 Q E L D T T G R D G N N D D T R N D A E L K R S T K S S L M G
H514 Q E L D T T G R D G N N D D T R S D A E L K R S T K S S L M G
H1114 Q E L D T T G R N G E N D D T R G D A E L K R S T K S S L M G
H1517 Q E L D T T G R N G E N D D T R G D A E L K R S T K S S L M G
I25 Q E L D T N G R D G N N D T T R N D A E L K R S T K S S L M G
I69 Q E L D T N G R N G N N D D T R N D A E L K R S T K S S L M G
I84 Q E L D T T G R N G E N D E T R R D A E L K R S T K S S L M G
I97 Q E L D T T G R N G E N D D T R G D A E L K R S T K S S L M G
I112 Q E L D T T G R N G E N D D T R G D A E L K R S T K S S L M G
I256 Q E L D T T G R N G E N D D T R G D A E L K R S T K S S L M G
a

RBS, receptor binding site.

b

The whole length of amino acid sequence of HA protein.

c

Key locations identified in this research where mutations contribute to the antigenic distance between clusters 1 and 2.

d

Locations holding mutations that contribute to the antigenic distance between vaccine strains and isolates and potential location under selection from vaccines.

e

The HA1 protein sequence without 10 resudies at the beginning of HA protein.