FIG 5.

The ability of ACE2 variants to facilitate entry of virus pseudotyped with coronavirus spike proteins. (A and B) HeLa cells transduced with lentiviruses expressing ACE2 orthologs, SNVs, or empty vector were infected with virus pseudotyped with SARS-CoV spike, SARS-CoV-2 spike, or VSV-G and containing a firefly luciferase (Fluc) reporter gene. Intracellular Fluc activity of cell lysates was determined after 48 h of infection. Values are means plus standard deviations (SD) (error bars) (n = 3). This experiment was independently repeated at least three times. **, P < 0.01; ***, P < 0.001. Significance assessed by one-way ANOVA.