Skip to main content
. 2022 Jan 12;96(1):e01492-21. doi: 10.1128/JVI.01492-21

FIG 6.

FIG 6

The ACE2 variants mediate authentic SARS-CoV-2 virus infection in vitro. (A) HeLa cells transduced with lentiviruses expressing human ACE2 SNVs or mouse ACE2 were infected with various doses of SARS-CoV-2 virus (MOI, 1, 0.3, 0.1, or 0.03). Expression of the viral nucleocapsid (N) protein or ACE2 orthologs was visualized by using the Operetta high content imaging system (PerkinElmer). Viral N protein (red) and ACE2 variant/ortholog (green) are shown. Scale bar, 100 μm. This experiment was independently repeated three times with similar results, and representative images are shown. (B) Images were analyzed and quantified using PerkinElmer Harmony high-content analysis software 4.9. The infection efficiency represents the percentage of SARS-CoV-2 infected cells/ACE2 positive cells (y axis). Values are means plus standard deviations (SD) (error bars) (n = 4). ns, no significance; **, P < 0.01. Significance assessed by one-way ANOVA.