FIG 2.

MIR2187HG decreases host antiviral immunity. (A) Schematic diagram of MIR2187HG expression plasmid construction (left) and the effect of the MIR2187HG-P expression plasmid on endogenous MIR2187HG expression (right). MICs were transfected with pcDNA3.1 vector or MIR2187HG-P expression plasmid for 48 h; then, MIR2187HG expression was determined by qPCR. (B) Location schematic diagram of si-MIR2187HG and screening of si-MIR2187HG efficiency. The six siRNAs of MIR2187HG were transfected into MICs for 48 h, and then MIR2187HG and miR-2187-3p expression was measured by qPCR after SCRV infection. (C and D) Effect of the expression plasmid of MIR2187HG-P or MIR2187HG-P-MT on TNF-α, IFN-2, Mx1, ISG15, and viperin expression during SCRV infection. MICs were transfected with pcDNA3.1 vector or MIR2187HG-P (C) or MIR2187HG-P-MT (D) expression plasmid for 24 h and then treated with SCRV for 6 h, 12 h, or 24 h; subsequently, expression of the indicated genes was determined by qPCR. (E) MICs were transfected with si-NC or si-MIR2187HG for 24 h. The cells were treated with SCRV for different times. Then, the expression of TNF-α, IFN-2, Mx1, ISG15, and viperin was analyzed by qPCR. (F and G) Cell proliferation was assessed by EdU assays in MICs transfected with pcDNA3.1 vector or MIR2187HG-P expression plasmid (F) and si-MIR2187HG or si-NC (G) to determine the effect of MIR2187 after SCRV infection. MICs were transfected with si-NC, si-MIR2187HG, pcDNA3.1 vector, or IRL for 24 h and then treated with SCRV for 24 h. A cell proliferation assay was performed. Bar, 20 μm. Values are means and SE from triplicate experiments. **, P < 0.01, and *, P < 0.05 compared to results with the control.