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. 2022 Jan 12;96(1):e01484-21. doi: 10.1128/JVI.01484-21

FIG 7.

FIG 7

lncRNA MIR2187HG promotes SCRV replication. (A) miR-2187-3p enhanced SCRV replication. MICs were transfected with NC, miR-2187-3p, NC-i, or miR-2187-3p-i for 24 h and then treated with SCRV (MOI = 5). After different SCRV treatment times, the intracellular SCRV level was determined by qPCR. (B) miR-2187-3p enhanced the cytopathic effect. MICs were transfected with NC, miR-2187-3p, NC-i, or miR-2187-3p-i for 24 h and infected with SCRV for 24 h. Then, cells were observed for morphological changes. (C) MIR2187HG promoted SCRV replication. MICs were transfected with pcDNA3.1, MIR2187HG-P, si-NC, or si-MIR2187HG for 24 h and then treated with SCRV (MOI = 5). After different SCRV treatment times, the intracellular SCRV level was determined by qPCR. (D) MIR2187HG enhanced cytopathic effect. MICs were transfected with pcDNA3.1, MIR2187HG-P, si-NC, or si-MIR2187HG for 24 h and infected with SCRV for 24 h. Then, cells were observed for morphological changes. (E) TBK1 inhibited SCRV replication. MICs were transfected with pcDNA3.1, TBK1, si-NC, or si-TBK1 for 24 h and then treated with SCRV (MOI = 5). After different SCRV treatment times, the intracellular SCRV level was determined by qPCR. (F) TBK1 enhanced the cytopathic effect. MICs were transfected with pcDNA3.1, TBK1, si-NC, or si-TBK1 for 24 h and infected with SCRV for 24 h. Then, cells were observed for morphological changes. All data are means and SE from at least three independent triplicate experiments. **, P < 0.01, and *, P < 0.05, versus the controls.