TABLE 1.
PCR primers
| Primer | Sequence (5′–3′) |
|---|---|
| MIR2187HG-RT-F | TCATCTTACGTTCCTCCTG |
| MIR2187HG-RT-R | AAGCCTCTATCAACTTCAATC |
| TBK1-qRT-F | AGAGCACCACCAACTACCT |
| TBK1-qRT-R | ACTTTGACGGCATACAGG |
| Mx1-qRT-F | GCTGCTTGTTTACTCCCA |
| Mx1-qRT-R | ACCTGCATCATCTCCCTC |
| ISG15-qRT-F | TGAACGGACAGAAGACGC |
| ISG15-qRT-R | TGAGGAATACCTGCATGG |
| miR-2187-3p-qRT-F | GCAGCGAACCATTATTTGC |
| miR-2187-3p-qRT-R | TCCAGTTTTTTTTTTTTTTTAAAGCAG |
| β-actin-qRT-F | GTGATGAAGCCCAGAGCA |
| β-actin-qRT-R | CGACCAGAGGCATACAGG |
| 5.8s-qRT-F | AACTCTTAGCGGTGGATCA |
| 5.8s-qRT-R | GTTTTTTTTTTTTTTTGCCGAGTG |
| Mx1-qRT-F | GCTGCTTGTTTACTCCCA |
| Mx1-qRT-R | ACCTGCATCATCTCCCTC |
| TNFα-qRT-F | CGCCACCACGCTCTTCTG |
| TNFα-qRT-R | GCCATTGGCCAGGAGGGC |
| IFN-2-qRT-F | GCTCTGCCTTCCCTGCTA |
| IFN-2-qRT-R | CAGTTGACTCCGCCCTCT |
| TBK1-3′UTR-WT-NheI-F | CTAGCTAGCAAGGAGGAGATGGAGGGAGT |
| TBK1-3′UTR-WT-XbaI-R | TGCTCTAGACCGGCAACAATGAAGTGAGT |
| TBK1-3′UTR-MT-F | AAAGGCTATATGCATGGTAAAATTATATCTGTCTGAACATAAATG |
| TBK1-3′UTR-MT-R | ACCATGCATATAGCCTTTAATTACCTTCACATGAGTTTATTAA |
| TBK1-KpnI-F | GACGACAAGAAGCTTGGTACCATGCAGAGCACCACCAACTACC |
| TBK1-XbaI-R | TATAGAATAGGGCCCTCTAGACGGCAACAATGAAGTGAGTAACA |
| GFP-TBK1-3′UTR-F | CGGGGTACCGCTAGAAGGAGGAGATGGAGGGAGT- |
| GFP-TBK1-3′UTR-R | CGCGGATCCCCGGCAACAATGAAGTGAGT |
| GFP-TBK1-3′UTR-MT-F | TATCGGCATCCACTCCATACGGTCTGCTTGGTCC |
| GFP-TBK1-3′UTR-MT-R | ATGGAGTGGATGCCGATACAAAAAGCTTCATCTCACAGTCG |
| NdiTBK1-3′UTR-WT-NheI F | CTAGCTAGCAGGATGGTGCCAAAGTA |
| NdiTBK1-3′UTR-WT-XhoI R | CCGCTCGAGAAGCCGTTAAAGTTCAG |
| NdiTBK1-3′UTR-MT-F | AAAGGGCTGATGTATGGTAAAATGGTATTTGTCTGAACAT |
| NdiTBK1-3′UTR-MT-R | ACCATACATCAGCCCTTTAATTACCTTCACATGAGTTCAT |
| LcrTBK1-3′UTR-WT-NheI F | TGTTTAAACGAGCTCGCTAGCCAGTTTAATTAGATTATTTAACTTTGACGTT |
| LcrTBK1-3′UTR-WT-XhoI R | CAGGTCGACTCTAGACTCGAGTAACTCAGTTATTGATTATTGATCGGC |
| LcrTBK1-3′UTR-MT-F | AAGGCTATATGCATGGGGAAATGACATTTGTCTG |
| LcrTBK1-3′UTR-MT-R | CCCATGCATATAGCCTTTAATTACCTTCACGAGTTTATTCA |
| MIR2187HG-HindIII-F | ACTATAGGGAGACCCAAGCTTTCATCTTACGTTCCTCCTGATGC |
| MIR2187HG-EcoRI-R | TGATGGATATCTGCAGAATTCAAGCCTCTATCAACTTCAATCCATT |
| MIR2187HG-MT-F | GTCAGCAATTCTGCCAGAATCAGCAATGCAATT |
| MIR2187HG-MT-R | TCTGGCAGAATTGCTGACATCACTAAAACAGGC |
| LcrMIR2187HG-HindIII-F | ACTATAGGGAGACCCAAGCTTTCATCTTACGTTCCTCCTGATGC |
| LcrMIR2187HG-EcoRI-R | TGATGGATATCTGCAGAATTCCCTCTATCAACTTCAATCCATTTTTG |
| NdiMIR2187HG-HindIII-F | ACTATAGGGAGACCCAAGCTTTCATCTTACGTTCCTCCTGATGC |
| NdiMIR2187HG-EcoRI-R | TGATGGATATCTGCAGAATTCAAGCCTCTATCAACTTCAATCCATT |