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. 2022 Jan 12;96(1):e01301-21. doi: 10.1128/JVI.01301-21

FIG 6.

FIG 6

Zika virus degradation and NS5 interaction require only the first two helices of the STAT2 CC domain. (A) Diagram of GFP-STAT2-NDCC indicating boundaries of 4 α-helices of the STAT2 CC domain. (B) Helices α3 and α4 are dispensable for NS5 interaction. Coimmunoprecipitation assay using the indicated GFP-STAT2-NDCC domain fragments along with FLAG-tagged NS5. (C) Zika virus induces degradation of GFP-STAT2 fragments 1 to 250 and 1 to 286. Immunoblotting was carried out as for Fig. 3C but using the indicated GFP fusion proteins. (D) Zika virus induces degradation of GFP-STAT2 fragments 1 to 250 and 1 to 286; fluorescence microscopy carried out as for Fig. 3D but using cells expressing the indicated GFP fusion proteins. Bar = 200 μm. Corresponding phase-contrast images are shown at the bottom. (E) Comparison of the amino acid sequence identity among human (Hu) and mouse (Mu) STAT2 and human STAT1 in the NDCC (aa 1 to 315) or the first two helices of the CC domain (aa 140 to 250).