Skip to main content
. 2022 Jan 12;96(1):e01500-21. doi: 10.1128/JVI.01500-21

FIG 5.

FIG 5

RNA-Seq analysis to identify QP509L/383R-deleted ASFV-regulated genes in PAMs at 24 h. (A) Volcano map of differentially expressed genes (DEGs) of DRG in ASFV- versus ASFV-ΔQP509L/383R-infected PAMs at 24 h. Red dots indicate significantly upregulated DEGs; green dots indicate significantly downregulated DEGs; gray dots indicate no significance. (B) Heat map of DEGs of DRG between ASFV- and ASFV-ΔQP509L/383R-infected PAMs at 24 h. (C) GO terms. (D) KEGG pathway enrichment. (E) Expression level of Ifnb, Traf2, Cxcl10, Isg15, Rantes, and Mx1 genes in ASFV-ΔQP509L/383R- and ASFV-infected PAMs by RNA-Seq analysis at 24 h. (F) Expression level of Ifnb, Traf2, Cxcl10, Isg15, Rantes, and Mx1 genes in ASFV-ΔQP509L/383R- and ASFV-infected PAMs by qPCR analysis. PAMs were infected with ASFV or ASFV-ΔQP509L/383R (multiplicity of infection [MOI]: 0.1) for 24 h. The samples were analyzed by qPCR (n = 3 per group; means ± SD). ERK, extracellular signal-regulated kinase; IL-17, interleukin-17; TGF-beta, transforming growth factor-beta; WT, wild type (ASFV).