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. 2022 Jan 12;96(1):e01343-21. doi: 10.1128/JVI.01343-21

FIG 1.

FIG 1

Epitope mapping of the sera from 5 early neutralizers. (A) Sera with antibodies against CD4bs were detected by ELISA with the following probes: resurfaced stabilized cores RSC3 and RSC3 P363N. Monoclonal antibodies 4E10 and VRC01 have been used as controls and are indicated by a blue box. (B) Sera with neutralizing antibodies against the MPER are detected by neutralization assays with viruses 7312A-C1 and 7312A. Monoclonal antibodies 2F5 and 4E10 have been used as controls and are indicated by a green box. (C) Sera with neutralizing antibodies against glycan-dependent motifs in the V2 and V3 loops are detected by neutralization assays with JRCSFwt and the corresponding N160K and N332A mutants (V2 and V3, respectively). Monoclonal antibodies 2G12 and PG9 have been used as controls and are indicated by an orange box.