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Comparative effect of SOS-T mutations in EN pseudoviruses. Virus stocks were obtained from cotransfection of 887-SOS-T and 936-SOS-T expression plasmids with an env-deficient HIV backbone (pNL4-3ΔenvFL) on HEK-293T cells, normalized for the amount of p24, and used to infect TZM-bl cells in triplicate. At 48 h postinfection, luciferase activity was evaluated and expressed as luciferase units (LU).
