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Env incorporation per VLP. VLPs were generated in 293F cells, and Env incorporation was analyzed by Western blotting in SDS-PAGE gels as indicated in Materials and Methods. (A) VLPs gp120 quantification with a rgp120 (Bal) standard curve. gp120 and p55 were detected with 447-52D and p24 (24-4) antibodies, respectively. (B) Relative Env (gp120) incorporation into VLPs. The ratios of gp120 to p55 were calculated by using phosphorimaging analysis.
