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. 2022 Jan 12;96(1):e01343-21. doi: 10.1128/JVI.01343-21

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Copyright © 2022 Beltran-Pavez et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Env-VLPs characterization. (A) Particle size distribution analyzed by NTA. (B) Percentage of VLPs that incorporated envelopes in a closed trimeric conformation determined by NTA after immunolabeling with DY-405-conjugated 35022 antibody. (C) Trimer detection analyzed by Western blotting in BN-PAGE gels. An AC10.29 rgp120 (gp120 monomer), a 96ZM651 SOSIP (trimer), and a JRFL-VLP (VLP-incorporated trimer) were used as controls. Trimers were detected with an α-gp120 and α-gp41 antibody cocktail. Trimers are indicated with asterisks.