Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-GAD65 | Millipore | Cat#AB1511 |
| Anti-GAD67 | Millipore | Cat#MAB5406 |
| Anti-TH | Millipore | Cat#AB152 |
| Alexa Fluor 568-conjugated goat anti-rabbit | Invitrogen | Cat#A11011 |
| Alexa Fluor 568-conjugated goat anti-chicken | Invitrogen | Cat#A11041 |
| Alexa Fluor 633-conjugated goat anti-chicken | Invitrogen | Cat#A21103 |
| Bacterial and Virus Strains | ||
| AAV5-EF1α-DIO-ChR2-eYFP | WUSTL Hope Center Viral Core | N/A |
| AAV2 retro-EF1a-DIO-eYFP | WUSTL Hope Center Viral Core | N/A |
| AAV5-EF1α-DIO-eYFP | WUSTL Hope Center Viral Core | N/A |
| AAV5-EF1α-DIO-ArchT-eGFP | WUSTL Hope Center Viral Core | N/A |
| AAV5-EF1α-DIO-Opto-β2AR-YFP | WUSTL Hope Center Viral Core | N/A |
| AAV5-FLEX-SaCas9-sgRNA-ADRB2-73-4 | WUSTL Hope Center Viral Core | N/A |
| AAV5-CaMKIIα-GcAMP6f | Penn Vector Core | N/A |
| AAV5-Syn-DIO-HM3D(Gq)-mCherry | Addgene | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Clozapine-N-Oxide (CNO) | Sigma-Aldrich | Cat#C0832 |
| Propranolol hydrochloride | Tocris Bioscience | Cat#0624 |
| Isoproterenol hydrochloride | Tocris Bioscience | Cat#1747 |
| VECTASHIELD Hardset Antifade Mounting Medium | Vector Laboratories | Cat#H-1400 |
| Critical Commercial Assays | ||
| RNAscope Fluorescent Multiplex Kit 2.0 | Advanced Cell Diagnostics | Cat#320850 |
| Wash Buffer Reagents | Advanced Cell Diagnostics | Cat#310091 |
| Protease III & IV Reagents | Advanced Cell Diagnostics | Cat#322340 |
| eGFP | Advanced Cell Diagnostics | Cat#400281 |
| Experimental Models: Organisms/Strains | ||
| Mouse: vGAT-IRES-Cre (Slc32a1tm2(cre)Lowl/J) | The Jackson Laboratory | Stock #016962 |
| Mouse: TH-IRES-Cre (Thtm1(cre)Te/Kieg) | Infrafrontier | EM:00254 |
| Mouse: vGlut1-IRES-Cre (Slc17a7tm1.1(cre)Hze/J) | Bred in house | Gift from Larry Zweifel |
| Mouse: Dbh-Cre (Dbhtm3.2(cre)Pjen/J) | Bred in house | Gift from Patricia Jensen (NIEHS) |
| Software and Algorithms | ||
| Image processing, cell counter module | ImageJ/Fiji | https://imagej.net/Fiji |
| Graphpad Prism 8.0 | GraphPad Software | https://www.graphpad.com/scientificsoftware/prism/ |
| Illustrator CS6 | Adobe | https://www.adobe.com/products/illustrator.html |
| EthoVision XT 10 | Noldus | https://www.noldus.com/animal-behavior-research/products/ethovision-xt |
| MATLAB | MathWorks | https://www.mathworks.com/ |
| Leica LAS X | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| Med-PC V Software Suite | Med-Associates, Inc. | https://www.med-associates.com/med-pc-v/ |
| Video Freeze | Med-Associates, Inc. | https://www.med-associates.com/product/video-fear-conditioning/ |
| nVista | Inscopix | https://www.inscopix.com/nVista |