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. 2022 Jan 12;8(2):eabk1789. doi: 10.1126/sciadv.abk1789

Fig. 5. RAD51AP2 is required for stabilizing recombination intermediates for efficient DSB repair in PARs.

Fig. 5.

(A) Representative spread early and mid-pachytene spermatocytes stained for SYCP3 (green) and MSH4 or TEX11 (red). (B) Frequencies of nuclei with MSH4 or TEX11 foci detected at the PAR in spread early and mid-pachytene spermatocytes with XY touching. NS, P > 0.05 and **P < 0.01, two-way ANOVA. (C to H) Representative spread spermatocytes stained for SYCP3 (green) and RAD51 (C) or RPA (F) (red), frequencies of nuclei with RAD51 (D) and RPA (G) foci detected at the PAR in spread mid- and late pachytene spermatocytes, and frequencies of nuclei with RAD51 (E) or RPA (H) foci detected at the PAR in spread Rad51ap2ΔC741/ΔC741 spermatocytes with XY touching or XY separated in late pachynema are shown. NS, P > 0.05 and *P < 0.05, two-way ANOVA. For (A), (C), and (F), magnified views of the X and Y chromosomes are shown on the right or above, with an asterisk indicating the PAR. Miniaturized H1t staining (white) images are shown in the left-lower corner of the overlay images. Scale bars, 10 μm. The boundaries of PARs were determined on the basis of the average ratios of PAR axial length to the total axial length of X or Y chromosomes (fig. S16). n, the number of spermatocytes scored from at least two mice per genotype. EP, early pachynema; MP, mid-pachynema; LP, late pachynema.