Table 1.
Primers used for qPCR experiments
| Gene | Primer name | Primer sequence | Product size (bp) | Efficiency (E, %) | Accession ID |
|---|---|---|---|---|---|
| c-fos | For Rev |
GTATTACCCGCTCAACCAGAC TCCAGTAACCCTCATTTTGGG |
200 pb | 99.1 | 394198 |
| egr-1 | For Rev |
AGTTTGATCACCTTGCTGGAG AACGGCCTGTGTAAGATATGG |
110 pb | 108.1 | 30498 |
| 18S | For Rev |
TCGCTAGTTGGCATCGTTTATG CGGAGGTTCGAAGACGATCA |
85 pb | 93 | 100037361 |
| emx-2 | For Rev |
GGACTCGTTTCGTTTCCTTG GGACTCGTTTCGTTTCCTTG |
199 pb | 94.4 | 30537 |
| emx-3 | For Rev |
TTCACTCCATCATCGGGTTC GCGTTTGACGAATTGGAGTC |
145 pb | 93.5 | 30536 |
| eomesa | For Rev |
CTTATTGATCTCCGCCTTGC TATTGGTGCTTTCGGAGGAC |
147 pb | 99.4 | 64603 |
| prox1a | For Rev |
TTACGAAGACGCTGTGATGC AATGGTGAAAGGCACTCCTG |
195 pb | 92.0 | 30679 |
| dlx2a | For Rev |
TTCAGCCACCACTTCATCAC AACAGTGTCACGCCCAAATC |
193 pb | 95.0 | 30574 |
| dlx5a | For Rev |
TCATACTCCACAGCGTATCACC AGTAAATGGTTCGGGGCTTC |
148 pb | 90.0 | 30569 |
Note: For, forward primer; Rev: reverse primer.