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. 2021 Nov 15;1(8):100118. doi: 10.1016/j.crmeth.2021.100118

Figure 7.

Figure 7

CUT&Tag-BS profiles low-cell-number samples

(A) H3K27me3-CUT&Tag-BS tracks at chr6: 48,879,717–55,974,833 generated with different numbers of input cells (250,000, 100,000, 20,000, and 4,000). CpG methylation status is indicated by color (red, methylated; blue, unmethylated).

(B) Venn diagrams showing overlap of peaks identified in 250,000 sample with those detected in 100,000, 20,000, or 4,000 sample.

(C) Density scatterplots displaying correlation of peak signals between 250,000 sample and samples with lower input cell numbers (100,000, 20,000, and 4,000). Each dot represents an individual peak in the unified peak set, with viridis color scale indicating density. Pearson's r value is shown at the top of each plot.