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Primer sequences of mRNA for RT-PCR analysis.
| Gene | Forward sequence (5′ to 3′) | Reverse sequence (5′ to 3′) | Product size/bp |
|---|---|---|---|
| Hif-1α | ACATCAAGTCAGCAACGTGGAAGG | GCAAGCATCCTGTACTGTCCTGTG | 379 |
| Pdk1 | ACTGCGACGTGGTGGAGGTC | ATGGTCGCTCTCATGGCATTCTTG | 202 |
| Glut1 | CAGTTCGGCTATAACACTGGTG | GCCCCCGACAGAGAAGATG | 156 |
| Ldha1 | TGTCTCCAGCAAAGACTACTGT | GACTGTACTTGACAATGTTGGGA | 155 |
| Hk2 | TGATCGCCTGCTTATTCACGG | AACCGCCTAGAAATCTCCAGA | 112 |
| Actb | TGATCGCCTGCTTATTCACGG | AACCGCCTAGAAATCTCCAGA | 205 |
