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TGF-β1 induced LX2 cells proliferation and fibrogenic gene expression. Cells were treated with TGF-β1 at 0-10 ng/mL for 24 h. (a) Effect of TGF-β1 on cell proliferation was measured by MTT. (B-D) The protein expression of COL I and α-SMA was analyzed by western blot assay. Data from the three independent trials were represented by mean ± S.D. ∗∗∗p <0.001 TGF-β1 vs control; ∗∗p <0.01 TGF-β1 vs control; ∗p <0.05 TGF-β1 vs control.
