Table 1.
Sequences of siRNAs used in the study. siRNA duplexes were designed with occasional G:U wobbles at the 5′ end of the antisense strand, as indicated by small lettered ‘u’. L1–3; leader-sequence specific siRNAs 1-3; O1–3, ORF1-specific siRNAs 1–3; N1–3, N-specific siRNAs 1–3; U, 3′UTR-specific siRNAs 1–3; GFP = Green Fluorescent Protein; Luc = Firefly Luciferase; A = adenine; C = cytosine; G = guanine; U = uracil; T = thymine
| Name | Sense strand (5′-3′) | Antisense strand (5′-3′) |
|---|---|---|
| L1 | UCUGUUCUCUAAACGAAuUTT | AGUUCGUUUAGAGAACAGAUC |
| L2 | CCAACCAACUUUCGAUuUuTT | GAGAUCGAAAGUUGGUUGGUU |
| L3 | AAACCAACCAACUUUCGAUTT | AUCGAAAGUUGGUUGGUUUGU |
| O1 | CCAAAUGUGCCUUUCAACUTT | AGUUGAAAGGCACAUUUGGUU |
| O2 | GUUACAUGCACCAUAUGGATT | UCCAUAUGGUGCAUGUAACAA |
| O3 | GGUACUUGGUAGUUUAGCUTT | AGCUAAACUACCAAGUACCAU |
| N1 | GAAUAAGCAUAUUGACGuATT | UGCGUCAAUAUGCUUAUUCAG |
| N2 | CAAAUUGGCUACUACCGAATT | UUCGGUAGUAGCCAAUUUGGU |
| N3 | CGCUUCAGCGUUCUUCGGAAUTT | AUUCCGAAGAACGCUGAAGCGTT |
| N4 | GGACGAUUGUUACGACGUUTT | AACGUCGUAACAAUCGUCCUA |
| N5 | CCCUUGAAGAGGACGAUuUTT | AGAUCGUCCUCUUCAAGGGGA |
| N6 | CGUGGGCGUUAGGACGAUUTT | AAUCGUCCUAACGCCCACGGU |
| N7 | GAUUGUUUCUGCCGUAGUATT | UACUACGGCAGAAACAAUCGU |
| N8 | GGGUGGUUGUCUCGGAUUUTT | AAAUCCGAGACAACCACCCUU |
| N9 | GUUCCUUGUUGUAACGGUUTT | AACCGUUACAACAAGGAACUC |
| N10 | GACGAUUGUUACGACGUUATT | UAACGUCGUAACAAUCGUCCU |
| N11 | CUAGUUCAGUAAAACGAuUTT | AGUCGUUUUACUGAACUAGAA |
| U1 | CUUUAAUCAGUGUGUAACATT | UGUUACACACUGAUUAAAGAU |
| U2 | CCUAAUGUGUAAAAUUAAUTT | AUUAAUUUUACACAUUAGGGC |
| U3 | CAUGUGAUUUUAAUAGCUUTT | AAGCUAUUAAAAUCACAUGGG |
| siGFP | GCAGCACGACUUCUUCAAGTT | CUUGAAGAAGUCGUGCUGCTT |
| siLuc | CGUACGCGGAAUACUUCGATT | UCGAAGUAUUCCGCGUACG |