Table 1.
Strains and plasmids used in this study
| Strains and plasmids | Description | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | hsdR recA lacZYAF80 lacZDM15 | BRL |
| BL21 (DE3) | F-dcm ompT hsdS (rB-mB-) gal λ(DE3) | Laboratory collection |
| S17-1 | F–recA hsdR RP4-2 (Tc::Mu)(Km::Tn7) lysogenized with λpir phage | Laboratory collection |
| DH5α/pUCP18/pDN19lacΩ-PpigA | E. coli DH5α containing plasmid pUCP18 and pDN19lacΩ-PpigA | This study |
| DH5α/pXW1903/pDN19lacΩ-PpigA | E. coli DH5α containing plasmid pXW1903 and pDN19lacΩ-PpigA | This study |
| DH5α/pUCP18/pDN19lacΩ-PSMWW4_v1c29280 | E. coli DH5α containing plasmid pUCP18 and pDN19lacΩ-PSMWW4_v1c29280 | This study |
| DH5α/pXW1903/pDN19lacΩ-PSMWW4_v1c29280 | E. coli DH5α containing plasmid pXW1903 and pDN19lacΩ-PSMWW4_v1c29280 | This study |
| S. marcescens strains | ||
| JNB5-1 | S. marcescens wild type strain | (10) |
| SK8-37 | psrA::GmR mutant of JNB5-1, prodigiosin producing mutant | This study |
| SK8-37/pXW1903 | Mutant SK8-37 containing plasmid pXW1903 | This study |
| SK8-37/pUCP18 | Mutant SK8-37 containing empty vector pUCP18 | This study |
| SK8-37/pXW2006 | Mutant SK8-37 containing plasmid pXW2006 | This study |
| ΔPsrA | psrA deleted mutant of S. marcescens JNB5-1 | This study |
| ΔPsrA/pXW1903 | Mutant ΔPsrA containing plasmid pXW1903 | This study |
| ΔPsrA/pUCP18 | Mutant ΔPsrA containing empty vector pUCP18 | This study |
| ΔTssE | tssE deleted mutant of S. marcescens JNB5-1 | This study |
| ΔTssE/pXW2002 | Mutant ΔTssE containing plasmid pXW2002 | This study |
| JNB5-1/pDN19lacΩ-PpigA | S. marcescens JNB5-1 containing plasmid pDN19lacΩ-PpigA | This study |
| SK8-37/pDN19lacΩ-PpigA | S. marcescens SK8-37 containing plasmid pDN19lacΩ-PpigA | This study |
| Plasmids | ||
| pRK2013Tn5G | Tn5G carrying plasmid, KmRGmR | (87) |
| pMD18T | Cloning vector, 2692 bp, AmpR, lacZ | TaKaRa |
| pET28a | E. coli expression vector, KmR | Laboratory collection |
| pET28a-PsrA | A derivative of pET-28a, harboring the psrA gene, KmR | This study |
| pXW1903 | psrA gene driven by Plac promoter cloned in pUCP18, ApR | This study |
| pUCP18 | Broad-host-range shuttle vector, ApR | (88) |
| pXW2002 | tssE gene driven by Plac promoter cloned in pUCP18, ApR | This study |
| pXW2006 | rhs2 gene driven by Plac promoter cloned in pUCP18, ApR | This study |
| pDN19lacΩ | Promoterless lacZ fusion vector, SpRSmRTcR | (89) |
| pDN19lacΩ-ppigA | pig operon promoter cloned in pDN19lacΩ, SpRSmRTcR | This study |
| pUTKm | Tn5-based delivery plasmid with KmRAmpR | (90) |