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. 2021 Dec 10;50(1):17–34. doi: 10.1093/nar/gkab1199

Figure 2.

Figure 2.

Dose dependant distribution of palmitic acid conjugated tcDNA in tissues following 12-week repeated dosing. (A) Schematic representation of the 12-week study protocol with different doses of palmitic acid conjugated tcDNA-ASO. Mdx mice were injected intravenously with 2, 4 or 10 μmol/kg/week of conjugated tcDNA (palm-tcDNA-PO or palm-tcDNA-PS) for 12 weeks and euthanized 2 weeks after the last dose for tissue collection. 2, 4 or 10 μmol/kg correspond to approximately 10, 20 and 50 mg/kg. (B) Quantification of palm-tcDNA in mouse serum collected 1 h post-injection by fluorescent hybridization assay (n = 4 per group; data are represented as mean ± SEM and ratio between the different doses for each compound are shown). (C) Quantification of tcDNA-ASO content in various tissues 2 weeks after the end of the 12-week dosing regimen by fluorescent hybridization assay. Results are expressed as mean ± SEM; n = 4 mice per group.