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. 2021 Dec 15;50(1):536–548. doi: 10.1093/nar/gkab1220

Table 3.

RNA-binding affinities (Kd /nM1) of FBF-2 and FBF-2/LST-1 measured by MST

FBF-2 FBF-2/LST-1A FBF-2/LST-1B wt FBF-2/LST-1B mutN FBF-2/LST-1B mutC
gld-1 FBEa 110 ± 21 79 ± 15 314 ± 59 110 ± 15 314 ± 62
cFBE 67 ± 13 52 ± 13 178 ± 56 85 ± 8 240 ± 63

1 K d values and standard deviations were determined from curves fit to three independently pipetted technical replicates.