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. 2021 Dec 16;225(5):810–819. doi: 10.1093/infdis/jiab595

Figure 1.

Figure 1.

Angiotensin-converting enzyme 2 (ACE2) expression on T cells is up-regulated during cell activation. A, Relative ACE2 messenger RNA (mRNA) levels in T cells determined with quantitative reverse-transcription polymerase chain reaction. B, Interleukin 2 (IL-2) levels released by cells following activation. C, ACE2 protein detected in T cells with immunoblot analysis. Image is representative of 3 biological replicates. Jurkat cells were activated with anti-CD3 and anti-CD28 and primary T cells with anti-CD3 antibodies. Alternatively, T cells were activated by phorbol myristate acetate (PMA) and ionomycin for 24 hours before analysis. Vero E6 cells were used as a positive control and Vero or Ramos-B cells as a negative control. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Error bars represent standard errors of the mean from biological replicates. Abbreviation: PBMCs, peripheral blood mononuclear cells.