TABLE 1.

Primers used in multiplex PCR for amplification of VT1, VT2, and eaeA gene sequences and in PCR inhibition studies

Primer	Oligonucleotide sequence (5′→3′)	Size of amplicon product (bp)	Reference
VT1 (SLT1)-F	ACA CTG GAT GAT CTC AGT GG	614	11
VT1 (SLT1)-R	CTG AAT CCC CCT CCA TTA TG
VT2 (SLT2)-F	CCA TGA CAA CGG ACA GCA GTT	779	11
VT2 (SLT2)-R	CCT GTC AAC TGA GCA CTT TG
eaeAO157:H7-F	AAG CGA CTG AGG TCA CT	450	22
eaeAO157:H7-R	ACG CTG CTC ACT AGA TGT
16S-F	AGA GTT TGA TCA TGG CTC AG	798	8
16S-R	GGA CTA CCA GGG TAT CTA AT